Model to compare binding capacities
An in vitro model was set-up by the Centre of Excellence in Mycotoxicology and Public Health at the university of Ghent (Belgium, MYTOX member) togetherwith Orffa to test different single compounds and mixtures fortheir binding capacity (%) of mycotoxins. Binding of the mycotoxin by the adsorbent is tested at pH3 and subsequent raised to pH?. The mycotoxin standard mixture tested consists of aflatoxins (AFB1, AFB2, AFG1, AFG2), fumonisins (FB1, FB2), zearalenone (ZEN), ochratoxin (OTA), deoxynivalenol (DON), HT-2 Toxin (HT-2), T-2 Toxin (T-2) and enniatin B (ENN B).
The model was applied to analyse a large number of ingredients. These can be divided into inorganic adsorbents, yeast-based adsorbents and organic adsorbents (non-yeast). Several compositions of ingredients were also screened. This screening resulted in the discovery of a best synergistic composition, consisting of 4 ingredients. The best synergistic composition is based on the binding properties of the zeolite, clinoptilolite, fortified with a specific phyllosilicate and complemented with yeast-derived and propionate compounds.
A benchmark study (Table 1) was set up using the developed in vitro model comparing the best synergistic composition (Excential Toxin Plus, ETP) to 8 premium mycotoxin management preparations (1-8). To make an assessment of the general binding capacity, the binding percentages obtained from the study are converted using the following legend: Complete binding (“+++”>90%), partial binding (“++”>50%; < 90%), limited binding (“+”>10%; <50%) and no significant binding (“0″<10%).
The majority of premium preparations show a complete binding of the aflatoxins tested, although some have difficulties binding AFG2 to the fullest extent. For fumonisins, all premium preparations show a clear negative pH effect. These premium preparations release the bound fumonisins to a certain extend at pH 7. In general, all premium preparation score badly regarding the binding of ZEN. This is in large contrast with the binding of enniatin B, where all premium preparations show complete binding at pH?. For ochratoxin, there is a big negative pH effect. Trichothecenes (DON, T-2 and HT-2) are in general difficult to bind. For DON, in particular, a large part is not bound by any preparation and detected in the supernatant by LCMS/MS. This suggests that biotransformation by any preparation into less toxic metabolites is minimal.
Considerations for feed formulators
Adsorbents can be the first line of defence inside the animal for some mycotoxins, but there should also be a focus on other defences for mycotoxins that are not easy to bind. Biotransformation of DON is becoming a popular term, but analyses of supernatant does not show effective reduction of DON levels in the premium mycotoxin management preparations tested. Propionate compounds can act as a preventive measure by supressing the growth of fungi during storage and this way reduce the production of mycotoxins. Binding is only one part of the solution.
Results from this benchmark study indicate that there are clear differences between premium mycotoxin management preparations and that Excential Toxin Plus can match the results of the best products in the market. Excential Toxin Plus was recently tested in vivo in broilers and laying hens and this in the African countries Senegal and Togo, respectively. The incorporation of the additive at standard preventive dosage in the broiler diet resulted in a beneficia! effect on performance. In the laying hens, the additive was able to positively influence the feed conversion rate, the egg production rate and support the egg weight and this in a mycotoxin contaminated diet. The positive effect was more pronounced in older birds. Excential Toxin Plus has also been evaluated recently on its adsorption capacity towards the alpha-toxin produced by Clostridium perfringens. lt seems that the product is highly capable of adsorbing the exotoxin and could therefore have a positive effect on the development of Clostridium perfringens-associated necrotic enteritis.
Excential Toxin Plus, manufactured by Orffa India, combines three binders with the activity of a mould inhibitor to offer a complete solution.
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